org.bdgenomics.adam.rdd.read

# AlignmentRecordRDDFunctions

#### class AlignmentRecordRDDFunctions extends ADAMSequenceDictionaryRDDAggregator[AlignmentRecord]

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ADAMSequenceDictionaryRDDAggregator[AlignmentRecord], Logging, Serializable, Serializable, AnyRef, Any
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1. AlignmentRecordRDDFunctions
2. ADAMSequenceDictionaryRDDAggregator
3. Logging
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### Instance Constructors

1. #### new AlignmentRecordRDDFunctions(rdd: RDD[AlignmentRecord])

### Value Members

1. #### final def !=(arg0: Any): Boolean

   Definition Classes
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2. #### final def ##(): Int

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3. #### final def ==(arg0: Any): Boolean

   Definition Classes
     AnyRef → Any

4. #### def adamAlignedRecordSave(args: ADAMSaveAnyArgs): Boolean

5. #### def adamBQSR(knownSnps: Broadcast[SnpTable], observationDumpFile: Option[String] = None, validationStringency: ValidationStringency = ValidationStringency.LENIENT): RDD[AlignmentRecord]

   Runs base quality score recalibration on a set of reads.

   Runs base quality score recalibration on a set of reads. Uses a table of known SNPs to mask true variation during the recalibration process.

   knownSnps

     A table of known SNPs to mask valid variants.

   observationDumpFile

     An optional local path to dump recalibration observations to.

   returns

     Returns an RDD of recalibrated reads.

6. #### def adamCharacterizeTagValues(tag: String): Map[Any, Long]

   Calculates the set of unique attribute values that occur for the given tag, and the number of time each value occurs.

   Calculates the set of unique attribute values that occur for the given tag, and the number of time each value occurs.

   tag

     The name of the optional field whose values are to be counted.

   returns

     A Map whose keys are the values of the tag, and whose values are the number of time each tag-value occurs.

7. #### def adamCharacterizeTags(): RDD[(String, Long)]

   Converts a set of records into an RDD containing the pairs of all unique tagStrings within the records, along with the count (number of records) which have that particular attribute.

   Converts a set of records into an RDD containing the pairs of all unique tagStrings within the records, along with the count (number of records) which have that particular attribute.

   returns

     An RDD of attribute name / count pairs.

8. #### def adamConvertToSAM(isSorted: Boolean = false): (RDD[SAMRecordWritable], SAMFileHeader)

   Converts an RDD of ADAM read records into SAM records.

   Converts an RDD of ADAM read records into SAM records.

   returns

     Returns a SAM/BAM formatted RDD of reads, as well as the file header.

9. #### def adamCountKmers(kmerLength: Int): RDD[(String, Long)]

   Cuts reads into _k_-mers, and then counts the number of occurrences of each _k_-mer.

   Cuts reads into _k_-mers, and then counts the number of occurrences of each _k_-mer.

   kmerLength

     The value of _k_ to use for cutting _k_-mers.

   returns

     Returns an RDD containing k-mer/count pairs.

   See also

     adamCountQmers

10. #### def adamFilterRecordsWithTag(tagName: String): RDD[AlignmentRecord]

    Returns the subset of the ADAMRecords which have an attribute with the given name.

    Returns the subset of the ADAMRecords which have an attribute with the given name.

    tagName

      The name of the attribute to filter on (should be length 2)

    returns

      An RDD[Read] containing the subset of records with a tag that matches the given name.

11. #### def adamFlagStat(): (FlagStatMetrics, FlagStatMetrics)

12. #### def adamGetReadGroupDictionary(): RecordGroupDictionary

    Collects a dictionary summarizing the read groups in an RDD of ADAMRecords.

    Collects a dictionary summarizing the read groups in an RDD of ADAMRecords.

    returns

      A dictionary describing the read groups in this RDD.

13. #### def adamGetSequenceDictionary(): SequenceDictionary

    Aggregates together a sequence dictionary from the different individual reference sequences used in this dataset.

    Aggregates together a sequence dictionary from the different individual reference sequences used in this dataset.

    returns

      A sequence dictionary describing the reference contigs in this dataset.

    Definition Classes
      ADAMSequenceDictionaryRDDAggregator

14. #### def adamMarkDuplicates(): RDD[AlignmentRecord]

15. #### def adamRePairReads(secondPairRdd: RDD[AlignmentRecord], validationStringency: ValidationStringency = ValidationStringency.LENIENT): RDD[AlignmentRecord]

    Reassembles read pairs from two sets of unpaired reads.

    Reassembles read pairs from two sets of unpaired reads. The assumption is that the two sets were _originally_ paired together.

    secondPairRdd

      The rdd containing the second read from the pairs.

    validationStringency

      How stringently to validate the reads.

    returns

      Returns an RDD with the pair information recomputed.

    Note

      The RDD that this is called on should be the RDD with the first read from the pair.

16. #### def adamRealignIndels(consensusModel: ConsensusGenerator = new ConsensusGeneratorFromReads, isSorted: Boolean = false, maxIndelSize: Int = 500, maxConsensusNumber: Int = 30, lodThreshold: Double = 5.0, maxTargetSize: Int = 3000): RDD[AlignmentRecord]

    Realigns indels using a concensus-based heuristic.

    Realigns indels using a concensus-based heuristic.

    isSorted

      If the input data is sorted, setting this parameter to true avoids a second sort.

    maxIndelSize

      The size of the largest indel to use for realignment.

    maxConsensusNumber

      The maximum number of consensus sequences to realign against per target region.

    lodThreshold

      Log-odds threhold to use when realigning; realignments are only finalized if the log-odds threshold is exceeded.

    maxTargetSize

      The maximum width of a single target region for realignment.

    returns

      Returns an RDD of mapped reads which have been realigned.

    See also

      RealignIndels

17. #### def adamSAMSave(filePath: String, asSam: Boolean = true, asSingleFile: Boolean = false, isSorted: Boolean = false): AnyVal

    Saves an RDD of ADAM read data into the SAM/BAM format.

    Saves an RDD of ADAM read data into the SAM/BAM format.

    filePath

      Path to save files to.

    asSam

      Selects whether to save as SAM or BAM. The default value is true (save in SAM format).

    isSorted

      If the output is sorted, this will modify the header.

18. #### def adamSAMString: String

19. #### def adamSave(args: ADAMSaveAnyArgs, isSorted: Boolean = false): Boolean

20. #### def adamSaveAsFastq(fileName: String, fileName2Opt: Option[String] = None, outputOriginalBaseQualities: Boolean = false, sort: Boolean = false, validationStringency: ValidationStringency = ValidationStringency.LENIENT, persistLevel: Option[StorageLevel] = None): Unit

    Saves reads in FASTQ format.

    Saves reads in FASTQ format.

    fileName

      Path to save files at.

    outputOriginalBaseQualities

      Output the original base qualities (OQ) if available as opposed to those from BQSR

    sort

      Whether to sort the FASTQ files by read name or not. Defaults to false. Sorting the output will recover pair order, if desired.

21. #### def adamSaveAsPairedFastq(fileName1: String, fileName2: String, outputOriginalBaseQualities: Boolean = false, validationStringency: ValidationStringency = ValidationStringency.LENIENT, persistLevel: Option[StorageLevel] = None): Unit

    Saves these AlignmentRecords to two FASTQ files: one for the first mate in each pair, and the other for the second.

    Saves these AlignmentRecords to two FASTQ files: one for the first mate in each pair, and the other for the second.

    fileName1

      Path at which to save a FASTQ file containing the first mate of each pair.

    fileName2

      Path at which to save a FASTQ file containing the second mate of each pair.

    validationStringency

      Iff strict, throw an exception if any read in this RDD is not accompanied by its mate.

22. #### def adamSingleReadBuckets(): RDD[SingleReadBucket]

    Groups all reads by record group and read name

    Groups all reads by record group and read name

    returns

      SingleReadBuckets with primary, secondary and unmapped reads

23. #### def adamSortReadsByReferencePosition(): RDD[AlignmentRecord]

24. #### final def asInstanceOf[T0]: T0

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25. #### def clone(): AnyRef

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26. #### final def eq(arg0: AnyRef): Boolean

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27. #### def equals(arg0: Any): Boolean

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28. #### def filterByOverlappingRegion(query: ReferenceRegion): RDD[AlignmentRecord]

    Calculates the subset of the RDD whose AlignmentRecords overlap the corresponding query ReferenceRegion.

    Calculates the subset of the RDD whose AlignmentRecords overlap the corresponding query ReferenceRegion. Equality of the reference sequence (to which these are aligned) is tested by string equality of the names. AlignmentRecords whose 'getReadMapped' method return 'false' are ignored.

    The end of the record against the reference sequence is calculated from the cigar string using the ADAMContext.referenceLengthFromCigar method.

    query

      The query region, only records which overlap this region are returned.

    returns

      The subset of AlignmentRecords (corresponding to either primary or secondary alignments) that overlap the query region.

29. #### def finalize(): Unit

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30. #### final def getClass(): Class[_]

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31. #### def getSequenceRecordsFromElement(elem: AlignmentRecord): Set[SequenceRecord]

    For a single RDD element, returns 0+ sequence record elements.

    For a single RDD element, returns 0+ sequence record elements.

    elem

      Element from which to extract sequence records.

    returns

      A seq of sequence records.

    Definition Classes
      AlignmentRecordRDDFunctions → ADAMSequenceDictionaryRDDAggregator

32. #### def hashCode(): Int

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33. #### final def isInstanceOf[T0]: Boolean

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34. #### def isTraceEnabled(): Boolean

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      Logging

35. #### def log: Logger

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36. #### def logDebug(msg: ⇒ String, throwable: Throwable): Unit

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37. #### def logDebug(msg: ⇒ String): Unit

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38. #### def logError(msg: ⇒ String, throwable: Throwable): Unit

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39. #### def logError(msg: ⇒ String): Unit

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40. #### def logInfo(msg: ⇒ String, throwable: Throwable): Unit

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41. #### def logInfo(msg: ⇒ String): Unit

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42. #### def logName: String

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43. #### def logTrace(msg: ⇒ String, throwable: Throwable): Unit

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44. #### def logTrace(msg: ⇒ String): Unit

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45. #### def logWarning(msg: ⇒ String, throwable: Throwable): Unit

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46. #### def logWarning(msg: ⇒ String): Unit

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47. #### def maybeSaveBam(args: ADAMSaveAnyArgs, isSorted: Boolean = false): Boolean

48. #### def maybeSaveFastq(args: ADAMSaveAnyArgs): Boolean

49. #### final def ne(arg0: AnyRef): Boolean

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50. #### final def notify(): Unit

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51. #### final def notifyAll(): Unit

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52. #### final def synchronized[T0](arg0: ⇒ T0): T0

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53. #### def toString(): String

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54. #### final def wait(): Unit

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      @throws( ... )

55. #### final def wait(arg0: Long, arg1: Int): Unit

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56. #### final def wait(arg0: Long): Unit

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### Inherited from ADAMSequenceDictionaryRDDAggregator[AlignmentRecord]

### Inherited from Logging

### Inherited from Serializable

### Inherited from AnyRef

### Inherited from Any

### Ungrouped